Calculate DNA oligo melting temperature

*  Sequence should contain only ACGTN base
*  “Nearest-neighbor method” is applied for Tm calculation
*  “Wallace rule” is automatically applied when the length of sequence is under 15 bases

Sequence
Na+ conc. (mM)
Mg2+ conc. (mM)
dNTP conc. (mM)
primer conc. (nM)
Tm (°C)
molecular weight
GC ratio (%)