Primer3Plus Version: 2.5.0
Primer3 Version : libprimer3 release 2.4.0
Source code is available at https://github.com/primer3-org.
We request but do not require that use of this software be cited in
publications as
Untergasser A, Cutcutache I, Koressaar T, Ye J, Faircloth BC, Remm M and Rozen SG.
Primer3--new capabilities and interfaces.
Nucleic Acids Res. 2012 Aug 1;40(15):e115.
The paper is available at
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3424584/
Source code available at https://github.com/primer3-org.
The development of primer3 is promoted by a small group of
enthusiastic scientists mainly in their free time.They do not gain
any financial profit with primer3.
There are two groups of primer3 users: end users, who run
primer3 to pick their primers and programmers, who use primer3
in their scripts or software packages. We encourage both to use
primer3.
If you are an end user, we request but do not
require that use of this software be cited in publications
as listed above under CITING PRIMER3.
If you are a programmer, you will see that primer3 is now
distributed under the GNU General Public License, version 2 or
(at your option) any later version of the License (GPL2).
As we understand it, if you include parts of the primer3 source
code in your source code or link to primer3 binary libraries in
your executable, you have to release your software also under
GPL2. If you only call primer3 from your software and interpret
its output, you can use any license you want for your software.
If you modify primer3 and then release your modified software,
you have to release your modifications in source code under
GPL2 as well.
We chose GPL2 because we wanted primer3 to evolve and for the
improvements to find their way back into the main distribution.
If you are programming a new web interface which runs primer3,
please include in the about page of the tool the sentence
"<your software name> uses primer3 version ...".
Please consider releasing your software under GPL2 as well,
especially if you do not want to maintain it in the future.
There is no need to ask us for permission to include primer3
in your tools.
Initial development of Primer3 was funded by Howard Hughes Medical
Institute and by the National Institutes of Health, National Human
Genome Research Institute under grants R01-HG00257 (to David C. Page)
and P50-HG00098 (to Eric S. Lander),
but ongoing development and maintenance are not currently funded.
Primer3 was originally written by Helen J. Skaletsky (Howard Hughes
Medical Institute, Whitehead Institute) and Steve Rozen (Duke-NUS
Graduate Medical School Singapore, formerly at Whitehead Institute)
based on the design of earlier versions, notably Primer 0.5
(Steve Lincoln, Mark Daly, and Eric S. Lander).
The original web interface was designed by Richard Resnick. Lincoln
Stein designed the Boulder-IO format in the days before XML and RDF, and
championed the idea of making primer3 a software component, which
has been key to its wide utility.
In addition, among others, Ernst Molitor, Carl Foeller, and James Bonfield
contributed to the early
design of primer3. Brant Faircloth has helped with
ensuring that primer3 runs on Windows and MacOS and with the
primer3 web site.
Triinu Koressaar and Maido Remm modernized the melting
temperature calculations in 2008.
Triinu Koressaar added secondary structure,
primer-dimer, and template mispriming based on a thermodynamic
model in 2.2.0.
Ioana Cutcutache is responsible for most of the
remaining improvements
in 2.2.0, including performance enhancements, modern
command line arguments, and new input tags to
control primer location (with the "overlap junction"
tags initially implemented by Andreas Untergasser).
Jian Ye patiently provided new requirements.
Harm Nijveen and Andreas Untergasser developed the webinterface
Primer3Plus in 2006-2009. Currently Primer3Plus is maintained by
Andreas Untergasser.
Primer3 is an open software development project hosted
on GitHub: https://github.com/primer3-org
.
Copyright (c) 1996,1997,1998,1999,2000,2001,2004,2006,2007,2008,2009,2010
2011,2012
Whitehead Institute for Biomedical Research, Steve Rozen
(http://purl.com/STEVEROZEN/), Andreas Untergasser and Helen Skaletsky. All rights
reserved.
This file is part of the primer3 suite and libraries.
The primer3 suite and libraries are free software;
you can redistribute them and/or modify them under the terms
of the GNU General Public License as published by the Free
Software Foundation; either version 2 of the License, or (at
your option) any later version.
This software is distributed in the hope that it will be useful,
but WITHOUT ANY WARRANTY; without even the implied warranty of
MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the
GNU General Public License for more details.
You should have received a copy of the GNU General Public License
along with this software (file gpl-2.0.txt in the source
distribution); if not, write to the Free Software
Foundation, Inc., 51 Franklin St, Fifth Floor, Boston, MA 02110-1301 USA
THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS
"AS IS" AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT
LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR
A PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL THE COPYRIGHT
OWNERS OR CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL,
SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT
LIMITED TO, PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES; LOSS OF USE,
DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY
THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT
(INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE
OF THIS SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE.