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Primer3Prefold
avoid secondary structures
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Source Code
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Sequence Id:
Paste template sequence below
Or upload sequence file:
Due to performance reasons a sequence can be maximally 2000 bp long. If the submitted sequence is bigger, select an included region < 2000 bp.
Included Region (start,length):
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Concentration of monovalent cations:
Opt Primer Tm:
Concentration of divalent cations:
Please visit
About
for Primer3 and Primer3Plus citation requests.
Primer3Prefold uses for folding the software UNAFold, a successor of mfold. License conditions require that you cite the following article if you use Primer3Prefold:
Markham, N. R. & Zuker, M. (2008) UNAFold: software for nucleic acid folding and hybriziation. In Keith, J. M., editor,
Bioinformatics, Volume II. Structure, Function and Applications
, number 453 in
Methods in Molecular Biology
, chapter 1, pages 3–31. Humana Press, Totowa, NJ. ISBN 978-1-60327-428-9.
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